Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Applications:

ELISA, WB, IHC

Name of antibody:

H2BC15

Immunogen:

Fusion protein of human H2BC15

Full name:

H2B clustered histone 15

Synonyms:

H2B/d; H2BFD; HIST1H2BN

SwissProt:

Q99877

IHC positive control:

Human breast cancer and Human lung cancer

IHC Recommend dilution:

50-300

WB Predicted band size:

14 kDa

WB Positive control:

Jurkat and K562 cell lysates

WB Recommended dilution:

200-1000