Background:

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively.

Applications:

ELISA, WB, IHC

Name of antibody:

PYGL

Immunogen:

Fusion protein of human PYGL

Full name:

phosphorylase, glycogen, liver

Synonyms：

GSD6

SwissProt:

P06737

ELISA Recommended dilution:

1000-2000

IHC positive control:

Human thyroid cancer and Human brain

IHC Recommend dilution:

25-100

WB Predicted band size:

97 kDa

WB Positive control:

PC3 cells and human fetal liver tissue

WB Recommended dilution:

200-1000