Background:

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Applications:

ELISA, WB

Name of antibody:

RAD17

Immunogen:

Fusion protein of human RAD17

Full name:

RAD17 checkpoint clamp loader component

Synonyms:

CCYC; R24L; RAD24; HRAD17; RAD17SP

SwissProt:

O75943

ELISA Recommended dilution:

1000-2000

WB Predicted band size:

77 kDa

WB Positive control:

Lovo cells

WB Recommended dilution:

200-1000