Background:

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Applications:

ELISA, WB, IHC

Name of antibody:

RAD50

Immunogen:

Synthetic peptide of human RAD50

Full name:

RAD50 double strand break repair protein

Synonyms:

NBSLD; RAD502; hRad50

SwissProt:

Q92878

ELISA Recommended dilution:

2000-10000

IHC positive control:

Human colon cancer and Human gastric cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

154 kDa

WB Positive control:

293T, Hela, K562, NIH/3T3 and RAW264.7 cells

WB Recommended dilution:

500-2000