Background:

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2.

Applications:

IHC

Name of antibody:

RAF1(Phospho-Thr269)

Immunogen:

Peptide sequence around phosphorylation site of threonine269 (S-T-T(p)-L-P) derived from Human C-RAF.

Full name:

Raf-1 proto-oncogene, serine/threonine kinase

Synonyms：

NS5; CRAF; Raf-1; c-Raf; CMD1NN

SwissProt:

P04049

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100