Background:

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Applications:

ELISA, IHC

Name of antibody:

TNFSF11

Immunogen:

Synthetic peptide corresponding to a region derived from 304-317 amino acids of human TNFSF11

Full name:

tumor necrosis factor superfamily member 11

Synonyms:

ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2

SwissProt:

O14788

ELISA Recommended dilution:

5000-10000

IHC Positive control:

Human esophagus cancer;Human brain

IHC Recommended dilution:

20-100