Background:

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus.

Applications:

WB, IHC

Name of antibody:

MAP3K8(Ab-400)

Immunogen:

Synthesized non-phosphopeptide derived from human MAP3K8 around the phosphorylation site of serine 400 (C-Q-S(p)-L-D).

Full name:

mitogen-activated protein kinase kinase kinase 8

Synonyms：

COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT

SwissProt:

P41279

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

53 kDa

WB Positive control:

COLO cells treated with Insulin (0.01U/ml, 15mins) lysate

WB Recommended dilution:

500-3000