中文名稱 ： V8蛋白酶S

其他中文名稱 ：蛋白酶S

英文名稱 ： Protease S Aureus

其他英文名稱 ： Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease

產品規格 ： BR，500u/mg

C A S ：66676-43-5

級 別 ：BR

來 源 ：Staph aureus V8

Activity ：≥500units/mg

活力定義 ：One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate

產品描述 ：Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).

性 狀 ：粉末。

用 途 ：生化研究。

保 存 ：2～8℃