Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Applications:

WB, IHC, IF

Name of antibody:

H2AFX

Immunogen:

Synthetic peptide of human H2AFX (Ab-139)

Full name:

H2A histone family, member X

Synonyms：

H2AX; H2A.X; H2A/X

SwissProt:

P16104

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

15 kDa

WB Positive control:

HepG2 and 293 cells

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200