Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Applications:

ELISA, WB, IHC

Name of antibody:

H4C1

Immunogen:

Fusion protein of human H4C1

Full name:

H4 clustered histone 1

Synonyms:

H4FA; HIST1H4A

SwissProt:

P62805

IHC positive control:

Human colon cancer and Human liver cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

11 kDa

WB Positive control:

LoVo, NIH/3T3 and 293T cells, mouse thymus tissue, Hela cells and mouse pancreas tissue

WB Recommended dilution:

500-2000