Background:

Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.

Applications:

ELISA, IHC

Name of antibody:

PYM1

Immunogen:

Synthetic peptide of human PYM1

Full name:

PYM homolog 1, exon junction complex associated factor

Synonyms:

PYM; WIBG

SwissProt:

Q9BRP8

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human brain

IHC Recommend dilution:

10-50