Background:

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Applications:

ELISA, WB, IHC

Name of antibody:

RAF1

Immunogen:

Fusion protein of human RAF1

Full name:

Raf-1 proto-oncogene, serine/threonine kinase

Synonyms：

NS5; CRAF; Raf-1; c-Raf; CMD1NN

SwissProt:

P04049

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human colon cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

73 kDa

WB Positive control:

Mouse muscle tissue and Hepg2 cell

WB Recommended dilution:

500-2000