Background:

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Applications:

WB, IHC

Name of antibody:

RB1(Phospho-Ser608)

Immunogen:

Peptide sequence around phosphorylation site of Serine 608(Y-L-S(p)-P-V) derived from Human Retinoblastoma.

Full name:

retinoblastoma 1

Synonyms：

RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

SwissProt:

P06400

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

106 kDa

WB Positive control:

Jurkat cells lysates

WB Recommended dilution:

500-1000