Background:

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Applications:

ELISA, WB, IHC

Name of antibody:

RCHY1

Immunogen:

Fusion protein of human RCHY1

Full name:

ring finger and CHY zinc finger domain containing 1

Synonyms:

ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996

SwissProt:

Q96PM5

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human esophagus cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

30 kDa

WB Positive control:

293T, A549, Jurkat, 231 cell lysates

WB Recommended dilution:

500-2000