Background:

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Applications:

WB, IHC, IF

Name of antibody:

RELA(phospho-Ser311)

Immunogen:

Peptide sequence around phosphorylation site of serine 311(F-K-S(p)-I-M) derived from Human NFκB-p65.

Full name:

v-rel avian reticuloendotheliosis viral oncogene homolog A

Synonyms：

p65; NFKB3

SwissProt:

Q04206

IHC positive control:

Human breast carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

60 kDa

WB Positive control:

Hela cells lysates untreated or treated with IFN-α

WB Recommended dilution:

500-1000

IF positive control:

Hela cells

IF Recommend dilution:

100-200