Background:

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

Applications:

WB, IHC, IF

Name of antibody:

TNK2(Phospho-Tyr284)

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1.

Full name:

tyrosine kinase, non-receptor, 2

Synonyms：

ACK; ACK1; ACK-1; p21cdc42Hs

SwissProt:

Q07912

IHC positive control:

Human breast carcinoma tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

115 kDa

WB Positive control:

HepG2 cells lysates treated with EGF

WB Recommended dilution:

500-1000

IF positive control:

A549 cells

IF Recommend dilution:

100-200