Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Applications:

WB, IHC, IF

Name of antibody:

TP53 (Phospho-Ser15)

Immunogen:

Synthetic peptide of human TP53 (Phospho-Ser15)

Full name:

tumor protein p53 (Phospho-Ser15)

Synonyms：

P53; BCC7; LFS1; TRP53

SwissProt:

P04637

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

44 kDa

WB Positive control:

293 cells treated with UV

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200