Background:

Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.

Applications:

ELISA, WB, IHC

Name of antibody:

TPPP

Immunogen:

Full length fusion protein

Full name:

tubulin polymerization promoting protein

Synonyms：

p24; p25; TPPP1; TPPP/p25; p25alpha

SwissProt:

O94811

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer and human thyroid cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

24 kDa

WB Positive control:

Human fetal brain tissue

WB Recommended dilution:

500-2000