Background:

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Applications:

WB, IHC

Name of antibody:

MAP3K5 (Phospho-Ser966)

Immunogen:

Synthetic peptide of human MAP3K5 (Phospho-Ser966)

Full name:

mitogen-activated protein kinase kinase kinase 5 (Phospho-Ser966)

Synonyms：

ASK1; MEKK5; MAPKKK5

SwissProt:

Q99683

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

155 kDa

WB Positive control:

293 and HepG2 cells treated with UV; 293 cells treated with serum and PMA; Cos7 and Hela cells treated with EGF; Hela cells treated with IFN-α

WB Recommended dilution:

500-1000