IF Recommended dilution

100-200

IF Positive control:

Hela cells

WB Recommended dilution:

500-1000

WB Positive control:

293 and Hela cell lysates

WB Predicted band size:

155 kDa

IHC Recommend dilution:

50-100

IHC positive control:

Human breast carcinoma

SwissProt:

Q99683

Synonyms:

ASK1; MEKK5; MAPKKK5

Full name:

mitogen-activated protein kinase kinase kinase 5

Immunogen:

Peptide sequence around aa.964-968 (S-I-S-L-P) derived from Human MAP3K5

Name of antibody:

MAP3K5(Ab-966)

Applications:

WB, IHC, IF

Background:

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.