Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Applications:

ELISA, WB, IHC

Name of antibody:

MAPK8

Immunogen:

Fusion protein of human MAPK8

Full name:

mitogen-activated protein kinase 8

Synonyms:

JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

SwissProt:

P45983

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human prostate cancer and Human liver cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

48 kDa

WB Positive control:

Jurkat, K562, Hela, A172 cell, Mouse brain tissue lysates

WB Recommended dilution:

500-2000