Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Applications:

ELISA, WB, IHC

Name of antibody:

MCM4

Immunogen:

Synthetic peptide of human MCM4

Full name:

Minichromosome maintenance complex component 4

Synonyms：

CDC21, CDC54, hCdc21, P1-CDC21

SwissProt:

P33991

ELISA Recommended dilution:

1000-5000

IHC positive control:

Human brain and Human breast cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

97 kDa

WB Positive control:

NIH/3T3, Hela and K562 cells

WB Recommended dilution:

500-2000