Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

Applications:

ELISA, WB, IHC

Name of antibody:

MCM6

Immunogen:

Fusion protein of human MCM6

Full name:

minichromosome maintenance complex component 6

Synonyms：

Mis5; P105MCM; MCG40308

SwissProt:

Q14566

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human cervical cancer and human tonsil

IHC Recommend dilution:

50-200

WB Predicted band size:

93 kDa

WB Positive control:

293T, K562, NIH/3T3 and hela cells

WB Recommended dilution:

500-2000