中文名稱 : 熒光素酶
其他中文名稱 : 熒光素酶(螢火蟲);重組型酵素
英文名稱 : Luciferase firefly
其他英文名稱 : Luciferase from Photinus pyralis (firefly)
產品規格 : BR,98%
C A S :61970-00-1
級 別 :BR
product line :BioUltra
Assay :≥98% (SDS-PAGE)
Mol wt :mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein :10-35% E1%/280
Measuring range :≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity :ATPase ≤5 nanomolar units/mg protein;Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定義 :One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
產品描述 :Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性 狀 :粉末。EC:1.13.12.7
用 途 :生化研究。
保 存 :-20℃。保質期1年