中文名稱 : V8蛋白酶S
其他中文名稱 :蛋白酶S
英文名稱 : Protease S Aureus
其他英文名稱 : Endoproteinase Glu-C from Staphylococcus aureus V8;V8 Protease
產(chǎn)品規(guī)格 : BR,500u/mg
C A S :66676-43-5
級(jí) 別 :BR
來 源 :Staph aureus V8
Activity :≥500units/mg
活力定義 :One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate
產(chǎn)品描述 :Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
性 狀 :粉末。
用 途 :生化研究。
保 存 :2~8℃